The high level of significance of the Rosario Chapel murals warrants a comprehensive strategy for their conservation and interpretation, and will require a collaborative process involving all stakeholders. The interpretation and presentation of the Rosario Chapel have the potential to tell a rich story of religion and culture brought to the island through Spanish colonialism and Afro-Caribbean influences and their subsequent transformation and adaptation to the unique perspective of Puerto Rican island culture. The study and stabilization of the mural paintings of the Capilla de la Virgen del Rosario offer important insight into the chapel's evolution and a number of recommendations for its future conservation and interpretation.